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gene was acquired by means of microscopic analysis of bacteria connected to glass protect slides (see Materials and Approaches
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The mutants were analyzed by inverse PCR, working with two oligonucleotides, OTn1 and OTn2, to amplify adjacent DNA fragment to your transposon insertion site, followed by sequencing in the cloned merchandise (see Components and Techniques
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Multiplication and the event of microcolonies separated by water-filled channels follow this celebration. The opportunity to form biofilm endows the germs with many essential qualities, like a marked boost in resistance to antibiotics (six).
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